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KMID : 0363320080290041083
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Journal of Korean Oriental Internal Medicine
2008 Volume.29 No. 4 p.1083 ~ p.1099
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The Effects of Bogimakseong-bang(補氣膜性方) Treatment on cBSA-induced Membranous Nephropathy in Mouse Model
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Lee Jung-Won
Cho Chung-Sik
Kim Cheol-Jung
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Abstract
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Objective: We aimed to identify the effects of Bogimakseong-bang(BGMSB) treatment on cBSA-induced MN in a mouse model.
Methods: We divided 20 mice into 4 groups. The normal group (NR) had no treatment. We used cBSA to induced MN to the other 3 groups. One group (CT) was treated with cBSA (7mg/kg i.p) only. The second (BG-250) was treated with cBSA (7mg/kg i.p) and BGMSB extract (250mg/kg, p.o). The third group (BG-500) was treated with cBSA(7mg/kg i.p) and BGMSB extract (500mg/kg, p.o). After cBSA and BGMSB extract treatment for 4 weeks, proteinuria, serum albumin, total cholesterol, serum creatinine, BUN, total nucleated spleen cell number and total infiltrated kidney cell number of all groups were measured. CD3e+/CD19+ and CD4+/CD8 cells ratio of peripheral blood, kidney and spleen of all groups were analyzed. IL-1β and TNF-α, IL-6, IgG, IgM, and IFN-γ levels of all groups were gauged. Histological analysis of kidney tissue and immunohistochemical staining (CD4 CD8) of kidneys were observed.
Results: Proteinuria significantly decreased and serum albumin increased in groups treated with cBSA and BGMSB extract compared with the control. Total cholesterol decreased but not significantly. CD3e+/CD19cells ratio of peripheral blood decreased. CD3e+/CD19+ and CD4+/CD8 cells percentage of kidney and spleen showed no significant change. Level of IL-1β, TNF-α and IL-6 significantly decreased. and IFN-γ increased but has not significantly. Concentration of IgG and IgM significantly decreased compared with control. Thickness of GBM decreased on histological analysis of kidney. Deposition of CD4 and CD8 decreased on immunohistochemical staining of kidney.
Conclusions: According to the above result, BGMSB had a significant effect for treating MN which is cBSA-induced.
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KEYWORD
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Bogimakseong-bang(補氣膜性方), membranous nephropathy, cationic bovine serum albumin(cBSA), GBM, proteinuria, serum albumin
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